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Estimating effective population size using RADseq: Effects of SNP selection and sample size ArchiMer
Marandel, Florianne; Charrier, Gregory; Lamy, Jean-baptiste; Le Cam, Sabrina; Lorance, Pascal; Trenkel, Verena.
Effective population size (Ne) is a key parameter of population genetics. However, Ne remains challenging to estimate for natural populations as several factors are likely to bias estimates. These factors include sampling design, sequencing method, and data filtering. One issue inherent to the restriction site‐associated DNA sequencing (RADseq) protocol is missing data and SNP selection criteria (e.g., minimum minor allele frequency, number of SNPs). To evaluate the potential impact of SNP selection criteria on Ne estimates (Linkage Disequilibrium method) we used RADseq data for a nonmodel species, the thornback ray. In this data set, the inbreeding coefficient FIS was positively correlated with the amount of missing data, implying data were missing...
Tipo: Text Palavras-chave: Effective population size; Linkage disequilibrium; NeEstimator; RADseq; Skates and rays.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00607/71934/70628.pdf
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Genomics and telemetry suggest a role for migration harshness in determining overwintering habitat choice, but not gene flow, in anadromous Arctic Char ArchiMer
Moore, Jean-sebastien; Harris, Les N.; Le Luyer, Jeremy; Sutherland, Ben J. G.; Rougemont, Quentin; Tallman, Ross F.; Fisk, Aaron T.; Bernatchez, Louis.
Migration is a ubiquitous life history trait with profound evolutionary and ecological consequences. Recent developments in telemetry and genomics, when combined, can bring significant insights on the migratory ecology of non-model organisms in the wild. Here, we used this integrative approach to document dispersal, gene flow and potential for local adaptation in anadromous Arctic Char from six rivers in the Canadian Arctic. Acoustic telemetry data from 124 tracked individuals indicated asymmetric dispersal, with a large proportion of fish (72%) tagged in three different rivers migrating up the same short river in the fall. Population genomics data from 6,136 SNP markers revealed weak, albeit significant, population differentiation (average pairwise FST =...
Tipo: Text Palavras-chave: Arctic; Conservation; Fish migration; Fishery management; Genotyping by sequencing; Population genomics; RADseq.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00409/52049/52719.pdf
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Seascape genomics reveals population isolation in the reef-building honeycomb worm, Sabellaria alveolata (L.) ArchiMer
Muir, Anna P.; Dubois, Stanislas; Ross, Rebecca E.; Firth, Louise B.; Knights, Antony M.; Lima, Fernando P.; Seabra, Rui; Corre, Erwan; Le Corguillé, Gildas; Nunes, Flavia.
ackground Under the threat of climate change populations can disperse, acclimatise or evolve in order to avoid fitness loss. In light of this, it is important to understand neutral gene flow patterns as a measure of dispersal potential, but also adaptive genetic variation as a measure of evolutionary potential. In order to assess genetic variation and how this relates to environment in the honeycomb worm (Sabellaria alveolata (L.)), a reef-building polychaete that supports high biodiversity, we carried out RAD sequencing using individuals from along its complete latitudinal range. Patterns of neutral population genetic structure were compared to larval dispersal as predicted by ocean circulation modelling, and outlier analyses and genotype-environment...
Tipo: Text Palavras-chave: RADseq; Ocean circulation modelling; Adaptation; Marine invertebrate; Larval dispersal.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00643/75538/76445.pdf
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The impact of aquaculture on the genetics and distribution of the onuphid annelid Diopatra biscayensis ArchiMer
Galaska, Matthew P.; Wethey, David S.; Arias, Andrés; Dubois, Stanislas; Halanych, Kenneth M.; Woodin, Sarah A..
Aim Evolutionary history of natural populations can be confounded by human intervention such as the case of decorator worm species Diopatra (Onuphidae), which have a history of being transported through anthropogenic activities. Because they build tubes and act as ecosystem engineers, they can have a large impact on the overall ecosystem in which they occur. One conspicuous member, Diopatra biscayensis, which was only described in 2012, has a fragmented distribution that includes the Bay of Biscay and the Normanno‐Breton Gulf in the English Channel. This study explores the origin of these worms in the Normanno‐Breton region, which has been debated to either be the result of a historic range contraction from a relic continuous population or a more recent...
Tipo: Text Palavras-chave: Bay of Biscay; Convex Hull; Onuphidae; Phylogeography; Population genetics; RADseq.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00692/80411/83522.pdf
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